Effect of Mannitol on the Pharmacokinetics of Amikacin in Wistar Rats

The study analyzed the effect of mannitol on the pharmacokinetics (PK) of amikacin. Adult Wistar rats were treated as follows: Group 1 (G1) received mannitol for three days, Group 2 (G2) received mannitol plus 10 mg/kg of amikacin simultaneously, and Group 3 only amikacin. The PK study was conducted on the 4th day. For which, blood samples were drawn at fixed times during 24 h and immunoenzymatically analyzed. Results revealed significant differences (p<0.05) between the groups, e.g. Cmax were 62.26 ± 15.75 μg/ml for G1, 72.63 ± 24.80 μg/ml for G2 and 68.61 ± 27.40 μg/ml for G3. The AUC also differed in the three groups, being largest for G2, 222.52 ± 47.30 μg/ml/h, and smallest for G1, 135.59 ± 39.00 μg/ml/h. Alteration of the PK parameters observed between the groups must be considered when both drugs are prescribed, although human studies are necessary to confirm the results.